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个人简历
教育经历
1999 - 2004 , 博士后 , 遗传学 , Stanford Unversity
1995 - 1999 , 理学博士 , 遗传学 , Michigan State University
1987 - 1991 , 理学学士 , 生物化学 , 北京大学
1995 - 1999 , 理学博士 , 遗传学 , Michigan State University
1987 - 1991 , 理学学士 , 生物化学 , 北京大学
工作经历
2019 - 至今 教授 (tenure), 北京大学生命科学学院
2016 - 至今 研究员, 北京未来基因诊断高精尖创新中心（ICG）
2015 - 至今 研究员, 北大-清华生命科学联合中心 （CLS）
2014 - 至今 研究员, 北京大学生物医学集成创新研究所（BIOPIC）
2007 - 至今 研究员, 北京大学生命科学学院，生化与分子生物学
2005 - 2007 Research Associate, 美国斯坦福大学医学院遗传学系
1999 - 2004 博士后, 美国斯坦福大学医学院遗传学系
2016 - 至今 研究员, 北京未来基因诊断高精尖创新中心（ICG）
2015 - 至今 研究员, 北大-清华生命科学联合中心 （CLS）
2014 - 至今 研究员, 北京大学生物医学集成创新研究所（BIOPIC）
2007 - 至今 研究员, 北京大学生命科学学院，生化与分子生物学
2005 - 2007 Research Associate, 美国斯坦福大学医学院遗传学系
1999 - 2004 博士后, 美国斯坦福大学医学院遗传学系
荣誉奖励
北京市先进工作者，2020
“昌聚工程”创业类人才，2020
2019年度中国医学重大进展奖，2019
北京市留学人员创新创业特别贡献奖，2019
BI Investigatorship Award, 2019
2018年度北京大学生命科学学院未名杰出科研奖, 2019
第四届北京大学产学研项目合作先进个人奖, 2019
北京市科学技术奖, 2018
中国科技部创新人才推进计划科技创新创业人才, 2018
北京大学教学优秀奖, 2018
谈家桢生命科学创新奖，2016
中国专利优秀奖，2016
科学中国人年度人物，2016
北京大学郑昌学教学优秀奖，2015
Bayer Investigator Award，2014
The Roche Chinese Young Investigator Award，2014
北京大学东宝奖教金，2012
北京大学生命科学学院最受欢迎教师奖，2010
“昌聚工程”创业类人才，2020
2019年度中国医学重大进展奖，2019
北京市留学人员创新创业特别贡献奖，2019
BI Investigatorship Award, 2019
2018年度北京大学生命科学学院未名杰出科研奖, 2019
第四届北京大学产学研项目合作先进个人奖, 2019
北京市科学技术奖, 2018
中国科技部创新人才推进计划科技创新创业人才, 2018
北京大学教学优秀奖, 2018
谈家桢生命科学创新奖，2016
中国专利优秀奖，2016
科学中国人年度人物，2016
北京大学郑昌学教学优秀奖，2015
Bayer Investigator Award，2014
The Roche Chinese Young Investigator Award，2014
北京大学东宝奖教金，2012
北京大学生命科学学院最受欢迎教师奖，2010
学术任职
北京大学生命科学学院学术委员会委员
北京大学第十届学位评定委员会生命科学类分会
北京大学交叉学科学术咨询专家委员会
哈尔滨工业大学基因编辑系统与技术工信部重点实验室学术委员会委员
出生缺陷遗传学研究北京市重点实验室学术委员会委员
启东产业创新基金专家评委
生命科学学院研究生教学委员会委员
中国药品注册审评专家咨询委员会委员
中国遗传学会基因组编辑分会副主任
中国生物化学与分子生物学会下属分子系统生物学专业委员会委员
中国生物化学学会北京分会理事
北京大学第十届学位评定委员会生命科学类分会
北京大学交叉学科学术咨询专家委员会
哈尔滨工业大学基因编辑系统与技术工信部重点实验室学术委员会委员
出生缺陷遗传学研究北京市重点实验室学术委员会委员
启东产业创新基金专家评委
生命科学学院研究生教学委员会委员
中国药品注册审评专家咨询委员会委员
中国遗传学会基因组编辑分会副主任
中国生物化学与分子生物学会下属分子系统生物学专业委员会委员
中国生物化学学会北京分会理事
杂志任职
Section Editor (Genome Editing and New Technology) for SCIENCE CHINA Life Sciences
科研领域描述
研究领域：
1. 基因编辑技术
2. 新型基因治疗及疫苗技术
3. 高通量功能基因组学
4. 重大疾病发生机制
1. 基因编辑技术
2. 新型基因治疗及疫苗技术
3. 高通量功能基因组学
4. 重大疾病发生机制
代表性论文
1. Qu L, Yi Z, Shen Y, Lin L, Chen F, Xu Y, Wu Z, Tang H, Zhang X, Tian F, Wang C, Xiao X, Dong X, Guo L, Lu S, Yang C, Tang C, Yang Y, Yu W, Wang J, Zhou Y, Huang Q, Yisimayi A, Liu S, Huang W, Cao Y, Wang Y, Zhou Z, Peng X, Wang J-W, Xie X, and Wei W*. (2022) Circular RNA Vaccines against SARS-CoV-2 and Emerging Variants. Cell (in press).
2. Liu Y, Ding B, Zheng L, Xu P, Liu Z, Chen Z, Wu P, Zhao Y, Pan Q, Guo Y, Wang W*, and Wei W*. (2022) Regulatory elements can be essential for maintaining broad chromatin organization and cell viability. Nucleic Acids Research (in press).
3. Li G, Li X, Zhuang S, Wang L, Zhu Y, Chen Y, Sun W, Wu Z, Zhou Z, Chen J*, Huang X*, Wang J*, Li D*, Li W*, Wang H*, and Wei W*. (2022). Gene editing and its applications in biomedicine. Sci China Life Sci 65, https://doi.org/10.1007/s11427-021-2057-0.
4. Yi Z, Qu L, Tang H, Liu Z, Liu Y, Tian F, Wang C, Zhang X, Feng Z, Yu Y, Yuan P, Yi Z, Zhao Y, and Wei W*. (2022) Engineered circular ADAR-recruiting RNAs increase the efficiency and fidelity of RNA editing in vitro and in vivo. Nat Biotechnol doi: 10.1038/s41587-021-01180-3.
5. Ding B, Liu Y, Liu Z, Zheng L, Xu P, Chen Z, Wu P, Zhao Y, Pan Q, Guo Y, Wei W*, and Wang W*. (2021) Non-coding loci without epigenomic signals can be essential for maintaining global chromatin organization and cell viability. Sci. Adv. 7(45), doi: 10.1126/sciadv.abi6020.
6. Zhou Z*, Zhang X, Lei X, Xiao X, Jiao T, Ma R, Dong X, Jiang Q, Wang W, Shi Y, Zheng T, Rao J, Xiang Z, Ren L, Deng T, Jiang Z, Dou Z, Wei W* and Wang J*. (2021) Sensing of cytoplasmic chromatin by cGAS activates innate immune response in SARS-CoV-2 infection. Sig Transduct Target Ther 6, 382. https://doi.org/10.1038/s41392-021-00800-3.
7. Zhu SY, Liu Y, Zhou Z., Xiao X, Liu ZH, Chen A, Dong XJ, Tian F, Chen SH, Xu YY, Wang CH, Li QH, Niu XR, Pan Q, Du S, Xiao JY*, Wang JW* and Wei W*. (2021). Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry. Sci China Life Sci. doi: 10.1007/s11427-021-1990-5.
8. Guo, S., Chen, Y., Liu, J., Zhang, X., Liu, Z., Zhou, Z., and Wei, W*. (2021). Low-density lipoprotein receptor-related protein 1 is a CROPs-associated receptor for Clostridioides difficile toxin B. Sci China Life Sci. doi: 10.1007/s11427-021-1943-9.
9. Xu, P., Liu, Z., Liu, Y., Ma, H., Xu, Y., Bao, Y., Zhu, S., Cao, Z., Wu, Z., Zhou, Z., and Wei, W*. (2021). Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs. Nat Biotechnol. 39(11): 1403-1413.
10. Liang YS, Zhang G*, Li QH, Han L, Hu XY, Guo Y, Tao WY, Zhao XM, Guo MZ, Gan TY, Tong YM, Xu YF, Zhou Z, Ding Q, Wei W*, and Zhong J*. (2021) TRIM26 is a critical host factor for HCV replication and determines host tropism. Sci. Adv. 7(2), doi:10.1126/sciadv.abd9732.
11. Liu Y, Liu Z, Cao Z, and Wei W*. (2020) Reply to: Fitness effects of CRISPR/Cas9-targeting of long noncoding RNA genes. Nat Biotechnol. 38(5): 577-578.
12. Liu L, Zhang Y, Liu M, Wei W, Yi C, and Peng J*. (2019) Structural insights into the specific recognition of 5-methylcytosine and 5-hydroxymethylcytosine by TAL effectors. Journal of Molecular Biology https://doi.org/10.1016/j.jmb.2019.11.023.
13. Zhang X, Yue D, Wang Y, Zhou Y, Liu Y, Qiu Y, Tian F, Yu Y, Zhou Z, and Wei W*. (2019) PASTMUS: mapping functional elements at single amino acid resolution in human cells. Genome Biology DOI: 10.1186/s13059-019-1897-7.
14. Qu L, Yi Z, Zhu S, Wang C, Cao Z, Zhou Z, Yuan P, Yu Y, Tian F, Liu Z, Bao Y, Zhao Y, Wei W*. (2019) Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs. Nat. Biotechnol. 37, 1059-1069.
15. X. Zhao, G. Zhang, X. Chen, L. Dai, X. Qu, S. Li, S. Liu, H. Song, Z. Gao, P. Yuan, Z. Liu, C. Li, Z. Shang, Y. Li, M. Zhang, J. Qi, H. Wang, N. Du, Y. Wu, Y. Bi, S. Gao, Y. Shi, J. Yan, Y. Zhang, Z. Xie*, W. Wei* and G. F. Gao*. (2019) Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B. Cell 177, 1553-1565.
16. Zhu S, Cao Z, Liu Z, He Y, Wang Y, Yuan P, Li W, Wei W*. (2019) Guide RNAs with embedded barcodes boost CRISPR-pooled screens. Genome Biol 20, 20, doi:10.1186/s13059-019-1628-0.
17. Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, and Wei W*. (2018) Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol 36, 1203-1210.
18. Zhang H, Zhou Y, Wang Y, Zhao Y, Qiu Y, Zhang X, Yue D, Zhou Z, and Wei W*. (2018). A surrogate reporter system for multiplexable evaluation of CRISPR/Cas9 in targeted mutagenesis. Sci Rep 8, 1042.
19. Zhang Y, Liu L, Guo S, Song J, Zhu C, Yue Z, Wei W* and Yi C*. (2017) Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition. Nat Commun 8(1): 901.
20. He R, Peng J, Yuan P, Yang J, Wu X, Wang Y and Wei W*. (2017). Glucosyltransferase Activity of Clostridium difficile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Sci Rep 7(1): 10532.
21. Zhou Y, Wang P, Tian F, Gao G, Huang L*, Wei W* and Xie X.S* (2017) Painting A Specific Chromosome with CRISPR/Cas9 for Live-cell Imaging. Cell Res. 27, 298-301.
22. Hu H, Zhang H, Wang S, Ding M, An H, Hou Y, Yang X, Wei W*, Sun Y*, Tang C*. (2017) Live visualization of genomic loci with BiFC-TALE. Sci. Rep. 7, 40192.
23. Zhou Y, Zhang H and Wei W*. (2016) Simultaneous generation of multi-gene knockouts in human cells. FEBS Letters 590, 4343-4353.
24. Zhu S, Li W, Liu J, Chen C-H, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu X* & Wei W*. (2016) Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR library. Nat Biotechnol 34, 1279-1286.
25. Peng J, Zhou Y, Zhu S and Wei W*. (2015) High-throughput Screens in Mammalian Cells Using the CRISPR-Cas9 System. FEBS Journal 282, 2089–2096.
26. He R, Peng J, Yuan P, Xu F, Wei W*. (2015) Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy 11:5, 740-747.
27. Ren Q, Li C, Yuan P, Cai C, Luo G, Zhang L and Wei W*. (2015) A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus. Sci. Rep. 5, 8865.
28. Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li S, Huang T, Feng H and Wei W*. (2015) Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Cell Res. 25:157-168.
29. Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y and Wei W*. (2014) High-throughput Screening of a CRISPR/Cas Library for Functional Genomics in Human Cells. Nature 509: 487-91.
30. Qian LL, Cai CZ, Yuan PF, Jeong SY, Yang XZ, Almeida VD, Ernst J, Costa M, Cohen SN, Wei WS*. (2014) Bidirectional effect of Wnt signaling antagonist DKK1 on the modulation of anthrax toxin uptake. Sci China Life Sci 57: 469–481.
31. Yang J, Zhang Y, Yuan P, Cai C, Ren Q, Guo S, Zhu C, Qi H and Wei W*. (2014) Complete Decoding of DNA Recognition by TAL Effector RVDs. Cell Res. 24:628-631.
2. Liu Y, Ding B, Zheng L, Xu P, Liu Z, Chen Z, Wu P, Zhao Y, Pan Q, Guo Y, Wang W*, and Wei W*. (2022) Regulatory elements can be essential for maintaining broad chromatin organization and cell viability. Nucleic Acids Research (in press).
3. Li G, Li X, Zhuang S, Wang L, Zhu Y, Chen Y, Sun W, Wu Z, Zhou Z, Chen J*, Huang X*, Wang J*, Li D*, Li W*, Wang H*, and Wei W*. (2022). Gene editing and its applications in biomedicine. Sci China Life Sci 65, https://doi.org/10.1007/s11427-021-2057-0.
4. Yi Z, Qu L, Tang H, Liu Z, Liu Y, Tian F, Wang C, Zhang X, Feng Z, Yu Y, Yuan P, Yi Z, Zhao Y, and Wei W*. (2022) Engineered circular ADAR-recruiting RNAs increase the efficiency and fidelity of RNA editing in vitro and in vivo. Nat Biotechnol doi: 10.1038/s41587-021-01180-3.
5. Ding B, Liu Y, Liu Z, Zheng L, Xu P, Chen Z, Wu P, Zhao Y, Pan Q, Guo Y, Wei W*, and Wang W*. (2021) Non-coding loci without epigenomic signals can be essential for maintaining global chromatin organization and cell viability. Sci. Adv. 7(45), doi: 10.1126/sciadv.abi6020.
6. Zhou Z*, Zhang X, Lei X, Xiao X, Jiao T, Ma R, Dong X, Jiang Q, Wang W, Shi Y, Zheng T, Rao J, Xiang Z, Ren L, Deng T, Jiang Z, Dou Z, Wei W* and Wang J*. (2021) Sensing of cytoplasmic chromatin by cGAS activates innate immune response in SARS-CoV-2 infection. Sig Transduct Target Ther 6, 382. https://doi.org/10.1038/s41392-021-00800-3.
7. Zhu SY, Liu Y, Zhou Z., Xiao X, Liu ZH, Chen A, Dong XJ, Tian F, Chen SH, Xu YY, Wang CH, Li QH, Niu XR, Pan Q, Du S, Xiao JY*, Wang JW* and Wei W*. (2021). Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry. Sci China Life Sci. doi: 10.1007/s11427-021-1990-5.
8. Guo, S., Chen, Y., Liu, J., Zhang, X., Liu, Z., Zhou, Z., and Wei, W*. (2021). Low-density lipoprotein receptor-related protein 1 is a CROPs-associated receptor for Clostridioides difficile toxin B. Sci China Life Sci. doi: 10.1007/s11427-021-1943-9.
9. Xu, P., Liu, Z., Liu, Y., Ma, H., Xu, Y., Bao, Y., Zhu, S., Cao, Z., Wu, Z., Zhou, Z., and Wei, W*. (2021). Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs. Nat Biotechnol. 39(11): 1403-1413.
10. Liang YS, Zhang G*, Li QH, Han L, Hu XY, Guo Y, Tao WY, Zhao XM, Guo MZ, Gan TY, Tong YM, Xu YF, Zhou Z, Ding Q, Wei W*, and Zhong J*. (2021) TRIM26 is a critical host factor for HCV replication and determines host tropism. Sci. Adv. 7(2), doi:10.1126/sciadv.abd9732.
11. Liu Y, Liu Z, Cao Z, and Wei W*. (2020) Reply to: Fitness effects of CRISPR/Cas9-targeting of long noncoding RNA genes. Nat Biotechnol. 38(5): 577-578.
12. Liu L, Zhang Y, Liu M, Wei W, Yi C, and Peng J*. (2019) Structural insights into the specific recognition of 5-methylcytosine and 5-hydroxymethylcytosine by TAL effectors. Journal of Molecular Biology https://doi.org/10.1016/j.jmb.2019.11.023.
13. Zhang X, Yue D, Wang Y, Zhou Y, Liu Y, Qiu Y, Tian F, Yu Y, Zhou Z, and Wei W*. (2019) PASTMUS: mapping functional elements at single amino acid resolution in human cells. Genome Biology DOI: 10.1186/s13059-019-1897-7.
14. Qu L, Yi Z, Zhu S, Wang C, Cao Z, Zhou Z, Yuan P, Yu Y, Tian F, Liu Z, Bao Y, Zhao Y, Wei W*. (2019) Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs. Nat. Biotechnol. 37, 1059-1069.
15. X. Zhao, G. Zhang, X. Chen, L. Dai, X. Qu, S. Li, S. Liu, H. Song, Z. Gao, P. Yuan, Z. Liu, C. Li, Z. Shang, Y. Li, M. Zhang, J. Qi, H. Wang, N. Du, Y. Wu, Y. Bi, S. Gao, Y. Shi, J. Yan, Y. Zhang, Z. Xie*, W. Wei* and G. F. Gao*. (2019) Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B. Cell 177, 1553-1565.
16. Zhu S, Cao Z, Liu Z, He Y, Wang Y, Yuan P, Li W, Wei W*. (2019) Guide RNAs with embedded barcodes boost CRISPR-pooled screens. Genome Biol 20, 20, doi:10.1186/s13059-019-1628-0.
17. Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, and Wei W*. (2018) Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol 36, 1203-1210.
18. Zhang H, Zhou Y, Wang Y, Zhao Y, Qiu Y, Zhang X, Yue D, Zhou Z, and Wei W*. (2018). A surrogate reporter system for multiplexable evaluation of CRISPR/Cas9 in targeted mutagenesis. Sci Rep 8, 1042.
19. Zhang Y, Liu L, Guo S, Song J, Zhu C, Yue Z, Wei W* and Yi C*. (2017) Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition. Nat Commun 8(1): 901.
20. He R, Peng J, Yuan P, Yang J, Wu X, Wang Y and Wei W*. (2017). Glucosyltransferase Activity of Clostridium difficile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Sci Rep 7(1): 10532.
21. Zhou Y, Wang P, Tian F, Gao G, Huang L*, Wei W* and Xie X.S* (2017) Painting A Specific Chromosome with CRISPR/Cas9 for Live-cell Imaging. Cell Res. 27, 298-301.
22. Hu H, Zhang H, Wang S, Ding M, An H, Hou Y, Yang X, Wei W*, Sun Y*, Tang C*. (2017) Live visualization of genomic loci with BiFC-TALE. Sci. Rep. 7, 40192.
23. Zhou Y, Zhang H and Wei W*. (2016) Simultaneous generation of multi-gene knockouts in human cells. FEBS Letters 590, 4343-4353.
24. Zhu S, Li W, Liu J, Chen C-H, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu X* & Wei W*. (2016) Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR library. Nat Biotechnol 34, 1279-1286.
25. Peng J, Zhou Y, Zhu S and Wei W*. (2015) High-throughput Screens in Mammalian Cells Using the CRISPR-Cas9 System. FEBS Journal 282, 2089–2096.
26. He R, Peng J, Yuan P, Xu F, Wei W*. (2015) Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy 11:5, 740-747.
27. Ren Q, Li C, Yuan P, Cai C, Luo G, Zhang L and Wei W*. (2015) A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus. Sci. Rep. 5, 8865.
28. Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li S, Huang T, Feng H and Wei W*. (2015) Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Cell Res. 25:157-168.
29. Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y and Wei W*. (2014) High-throughput Screening of a CRISPR/Cas Library for Functional Genomics in Human Cells. Nature 509: 487-91.
30. Qian LL, Cai CZ, Yuan PF, Jeong SY, Yang XZ, Almeida VD, Ernst J, Costa M, Cohen SN, Wei WS*. (2014) Bidirectional effect of Wnt signaling antagonist DKK1 on the modulation of anthrax toxin uptake. Sci China Life Sci 57: 469–481.
31. Yang J, Zhang Y, Yuan P, Cai C, Ren Q, Guo S, Zhu C, Qi H and Wei W*. (2014) Complete Decoding of DNA Recognition by TAL Effector RVDs. Cell Res. 24:628-631.
实验室简介
课题组致力于发展基因编辑技术、新型基因治疗及疫苗技术、以及高通量功能基因组学技术，并关注癌症、感染等重大疾病的分子机制研究，为发展高效治疗手段提供新的药物靶点和思路。
